Long-lifetime LabelsSETA BioMedicals

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Long-lifetime Labels for measurement of high molecular-weight analytes (20 – 80 kDa) in a fluorescence polarization assay (FPIA)

If you require help with selecting your lifetime labels for a specific application click here.

 

Click product number to get the data sheet in PDF format
 
Number Product Name Target
Group		 Laser
Excitation [nm]		 Solvent
System1		 Absorption
max.
[nm]		 Extinction
Coefficient
[M–1cm–1]		 Emission
max.
[nm]		 Fluorescence
Lifetime [ns]
380 405 Free BSA2
K1-204
SeTau-380-NHS
 NH2 + + water 381 1,500 480 32.5 30.6
K4-1082
SeTau-270-NHS
 NH2     water 272 10,800 385 10.2 11.7
K7-545
SeTau-425-NHS
 NH2   + water 425 4,200 545 26.2  
K7-547
TG-404-NHS
 NH2 + + water 405 13,800 518 9.3 7.6
K7-549
SeTau-404-NHS
 NH2 + + water 402 10,600 515 9.3 8.8

Advantages of SeTau dyes:

  • Large Stokes shifts in the order of 100 nm
  • Highly water-soluble
  • Highly photostable
  • Low Quenching when labeled to proteins

 

Lifetime Labels for Biomedical Applications

Click product number to get the data sheet in PDF format
 
Number Product Name Target
Group		 Laser
Excitation [nm]		 Solvent
System1		 Absorption
max.
[nm]		 Extinction
Coefficient
[M–1cm–1]		 Emission
max.
[nm]		 Fluorescence
Lifetime [ns]		 Applications3
380 405 488 532 635 670 Free BSA2(D/P)
K8-1252
Square-635-di-NHS
 NH2         +   PB 7.4 636 216,000 648 0.27 2.44 (0.6)
RET FLT PL DNA Ol
K8-1320
Square-670-di-NHS
 NH2   +       + PB 7.4 667 188,000 685 0.54 3.26 (1.0)
RET FLT PL DNA Ol
K8-1352
Square-660-mono-NHS
 NH2   +       + PB 7.4 658 182,000 677 0.26 3.32 (1.2)
RET FLT PL DNA Ol
K8-1367
Seta-670-NH-di-NHS
 NH2   +       + PB 7.4 672 93,000 696 2.00 1.80
RET FLT PL DNA Ol pH
K8-1626
Seta-633-di-NHS
 NH2         +   PB 7.4 635 192,000 644 0.70 3.00
RET FLT PL DNA Ol
K8-1667
Seta-635-NH-di-NHS
 NH2         +   PB 7.4 640 188,000 656 1.80 1.37 (2.0)
RET FLT PL DNA Ol pH

 

1 Solvent system:
3 Applications:
  • PB 7.4 - Phosphate buffer, pH 7.4
  • CHCl3 - Chloroform

 

2 BSA conjugated dye
  • RET - Resonance Energy Transfer
  • FLT - Fluorescence Lifetime-based Applications
  • FP - Fluorescence Polarization-based Applications
  • PL - Protein Labeling
  • DNA - Labeling of DNA
  • OL - Labeling of Oligonucleotides
  • Pep - Labeling of peptides
  • L - Labeling of Lipids
  • pH - pH-Sensitive

 

 

Calculation of the Dye-to-Protein Ratio

Frequently Used Fluorescence Excitation Sources

 

 

Last modified: 01/17/10
 
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