Reactive LabelsSETA BioMedicals

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Amine- and Thiol-Reactive Fluorescent Labels for covalent attachment to biomolecules (proteins, amino-acids, peptides, oligonucleotides, lipids, and DNA)

Reactive dye selection chart

Label Absorption
max.
[nm]		 Emission
max.
[nm]		 Excellent Alternative to: Applications1 and Advantages Excitation
TG-404
 405 518
Alexa 405, Pacific Blue, Cascade Blue
FP, FLT, RET
405 diode laser
SeTau-425
 425 545
Proprietary label
FP, FLT, RET
405 or 430 diode lasers
Seta-632
 637 647
Alexa 633, DyLight 633
Labeling of proteins
He-Ne 633 laser and 635 diode laser
Seta-633
 637 647
Alexa 633
Labeling of proteins
He-Ne 633 laser and 635 diode laser
Seta-635
 639 655
Alexa 633, DyLight 633, HiLight 633
Labeling of oligos and small molecules
He-Ne 633 laser and 635 diode laser
Seta-646
 646 656
Alexa 647, Cy5, Allophycocyanin
Labeling of proteins
Kr-Ar 647 laser and 635 diode laser
Seta-660
 661 672
Alexa 660, Cy5.5
Labeling of proteins
Kr-Ar 647 laser and 635 diode laser
Seta-670
 667 686
Alexa 680, Cy5.5
Labeling of proteins
Kr-Ar 647 laser, 635 and 670 diode lasers
Square-635
 647 666
Cy5, Alexa 647
Imaging, extremely photostable
Kr-Ar 647 laser and 635 diode laser
Square-650-pH
 662 677
pHrhodo, CypHer
Imaging, pH-sensitive
Kr-Ar 647 laser and 635 diode laser

If you require more help with selecting your label click here.

Click product number to get the data sheet in PDF format
 
Number Product Name Target
Group		 Laser
Excitation [nm]		 Solvent
System2		 Absorption
max.
[nm]		 Extinction
Coefficient
[M–1cm–1]		 Emission
max.
[nm]		 Fluorescence
Lifetime [ns]		 Applications1
380 405 488 532 635 670 Free BSA3
K1-204
SeTau-380-NHS
 NH2 + +         water 381 1,500 480 32.5 30.6
FLT FP PL DNA OL
K5-3212
5-(and-6)-Carboxytetraethyl-
rhodamine, inner salt, NHS
 NH2       +     water 556 72,000 582    
FLT FP PL DNA OL Pep
K5-3222
5-(and-6)-Carboxy-X-rhodamine,
inner salt, NHS
 NH2       +     water 578 90,000 602    
FLT FP PL DNA OL Pep
K8-1342
Seta-670-mono-NHS
 NH2   +     + + PB 7.4 667 179,000 688   2.4
RET FLT PL DNA OL
K8-1352
Square-660-mono-NHS
 NH2   +       + PB 7.4 658 182,000 677 0.29 3.09
RET FLT PL DNA OL
K8-1357
Square-685-mono-NHS
 NH2 + +     + + CHCl3 683 208,000 703    
RET FLT Ol L
K8-1407
Square-650-pH-mono-NHS
 NH2         +   PB 7.4 653 135,000 671    
RET FLT PL DNA OL L pH
K8-1626
Seta-633-di-NHS
 NH2         +   PB 7.4 635 192,000 644 0.70 3.00
RET FLT PL DNA OL
K8-1641
Seta-632-mono-Maleimide
 SH         +   PB 7.4 633 270,000 642    
RET FLT PL DNA OL
K8-1642
Seta-632-mono-NHS
 NH2         +   PB 7.4 632 280,000 641    
RET FLT PL DNA OL
K8-1644
Seta-635-mono-NHS
 NH2         +   PB 7.4 634 181,000 644    
RET FLT PL DNA OL
K8-1663
Seta-633-mono-NHS
 NH2         +   PB 7.4 633 250,000 644    
RET FLT PL DNA OL pH
K8-1664
Seta-640-NH-mono-Maleimide
 SH         +   PB 7.4 640 160,000 656    
RET FLT PL DNA OL pH
K8-1667
Seta-635-NH-di-NHS
 NH2         +   PB 7.4 640 188,000 656 1.80 1.40
RET FLT PL DNA OL pH
K8-1669
Seta-635-NH-mono-NHS
 NH2         +   PB 7.4 638 136,000 655    
RET FLT DNA OL pH
K8-1672
Seta-646-mono-NHS
 NH2         +   PB 7.4 646 207,000 656    
RET FLT DNA OL
K8-1682
Seta-660-di-NHS
 NH2         +   PB 7.4 661 151,000 672    
RET FLT PL DNA OL
K8-3002
Seta-470-mono-NHS
 NH2 + + +       PB 7.4 469 50,000 521    
RET FLT PL DNA OL
                             
 
  Available on Request:                            
                               
K4-1082
SeTau-270-NHS
 NH2             water 272 10,800 385 10.2 11.7
FLT FP PL DNA OL Pep
K7-545
SeTau-425-NHS
 NH2   +         water 425 4,200 545 26.2  
FLT FP PL DNA OL Pep
K7-547
TG-404-NHS
 NH2 + +         water 405 13,800 518 9.3 7.6
FLT FP PL DNA OL
K7-549
SeTau-404-NHS
 NH2 + +         water 402 10,600 515 9.3 8.8
FLT FP PL DNA OL Pep
K8-1252
Square-635-di-NHS
 NH2         +   PB 7.4 636 216,000 648 0.26 2.44
RET FLT PL DNA OL
K8-1261
Square-650-mono-NHS
 NH2 + +     +   CHCl3 649 138,000 664    
RET FLT OL L
K8-1262
Seta-635-di-NHS
 NH2   +     +   PB 7.4 640 120,000 653 0.5  
RET FLT PL DNA OL
K8-1320
Square-670-di-NHS
 NH2   +       + PB 7.4 667 188,000 685 0.46 3.26
RET FLT PL DNA OL
K8-1344
Seta-665-mono-NHS
 NH2   +     + + PB 7.4 667 118,000 683   3.0
RET FLT PL DNA OL
K8-1367
Seta-670-NH-di-NHS
 NH2   +       + PB 7.4 672 93,000 696 2.00 1.80
RET FLT PL DNA OL pH
K8-1377
Seta-690-NH-di-NHS
 NH2   +       + PB 7.4 693 113,000 714   1.5
RET FLT PL DNA OL pH
K8-1382
Seta-660-ammonium-NHS
 NH2   + +   + + PB 7.4 658 150,000 678    
RET FLT PL DNA OL
K8-1446
Seta-700-mono-NHS
 NH2 + +     + + CHCl3 702 54,000 728    
RET FLT  OL L
K8-1752
Square-635-B-di-NHS
 NH2   + +   +   PB 7.4 628 103,000 649    
RET FLT PL DNA OL
K8-1762
Seta-635-B-di-NHS
 NH2   +     +   PB 7.4 634 131,000 656 0.5 3.0
RET FLT PL DNA OL
K8-1767
Seta-635-B-NH-di-NHS
 NH2   +     +   PB 7.4 641 87,000 670   2.1
RET FLT PL DNA OL pH
K8-1777
Seta-660-B-NH-di-NHS
 NH2   +     + + PB 7.4 662 87,000 684   1.5
RET FLT PL DNA OL pH
K8-1782
Seta-660-B-di-NHS
 NH2   +     + + PB 7.4 659 132,000 679   2.2
RET FLT PL DNA OL

 

1 Applications:
2 Solvent system:
  • RET - Resonance Energy Transfer
  • FLT - Fluorescence Lifetime-based Applications
  • FP - Fluorescence Polarization-based Applications
  • PL - Protein Labeling
  • DNA - Labeling of DNA
  • OL - Labeling of Oligonucleotides
  • Pep - Labeling of peptides
  • L - Labeling of Lipids
  • pH - pH-Sensitive
  • PB 7.4 - Phosphate buffer, pH 7.4
  • CHCl3 - Chloroform

 

3 BSA conjugated dye

 

Calculation of the Dye-to-Protein Ratio

Frequently Used Fluorescence Excitation Sources

 

 

Last modified: 01/02/09
 
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