Fluorescent ProbesSETA BioMedicals

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Environmentally sensitive Non-Reactive Fluorescent Probes

K35 — Fluorescent probe for albumin binding sites in plasma and serum
K37 (K6-1039) — Cholesterol sensitive probe
K8-Series — Protein sensitive probes
 
Click product number to get the data sheet in PDF format
 
Number Product Name Solvent
System
1
Absorption
max.
[nm]
Extinction 
Coefficient
[M–1cm–1]
Emission
max.
[nm]
QY
[%]
FLT
[ns]
Solvent
System
1
Absorption
max.
[nm]
Emission
max.
[nm]
QY
[%]
FLT
[ns]
K35
K35
Ethanol 430 13,200 no < 0.1 Toluene 410 495 60  
K6-207
3-DAB
PB 7.4 455 8,6002 700 2 1.5 PB + BSA 6 mg/mL 465 637 24 6.5
K6-1037
K37
Ethanol 449 29,5003 630 0.7   Toluene 434 540 49  
K8-1351
Square-660-mono-carboxy
PB 7.4 658 182,000 678 3   PB + BSA 6 mg/mL 679 696 24  
K8-1380
Seta-660-ammonium
PB 7.4 658 150,000 678 2   PB + BSA 6 mg/mL 685 701 34  

1 Solvent system:

2 Ethanol
3 Toluene

Frequently Used Fluorescence Excitation Sources

Biological Imaging

Dog spermatozoa stained with a mixture of K35 and K8-1400 fluorescent probes (450–490-nm band-pass excitation filter and 500-nm cutoff emission filter were used) Dog spermatozoa stained with a mixture of K35 and K8-1400 fluorescent probes (545–580-nm band-pass excitation filter and 610–800-nm band-pass emission filter were used)
   
Dog spermatozoa stained with K35 fluorescent probe (450–490-nm band-pass excitation filter and 500-nm cutoff emission filter were used) Dog spermatozoa stained with a mixture of K8-1412 fluorescent probes (595–650-nm band-pass excitation filter and 670–740-nm band-pass emission filter were used)

 

 

Last modified: 01/02/09
 
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