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Fluorescent Probes |
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| Number | Product Name | Solvent System1 |
Absorption max. [nm] |
Extinction Coefficient [M–1cm–1] |
Emission max. [nm] |
QY [%] |
FLT [ns] |
Solvent System1 |
Absorption max. [nm] |
Emission max. [nm] |
QY [%] |
FLT [ns] |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
K35 |
K35 |
Ethanol | 430 | 13,200 | no | < 0.1 | — | Toluene | 410 | 495 | 60 | |
K6-207 |
3-DAB |
PB 7.4 | 455 | 8,6002 | 700 | 2 | 1.5 | PB + BSA 6 mg/mL | 465 | 637 | 24 | 6.5 |
K6-1037 |
K37 |
Ethanol | 449 | 29,5003 | 630 | 0.7 | Toluene | 434 | 540 | 49 | ||
K8-1351 |
Square-660-mono-carboxy |
PB 7.4 | 658 | 182,000 | 678 | 3 | PB + BSA 6 mg/mL | 679 | 696 | 24 | ||
K8-1380 |
Seta-660-ammonium |
PB 7.4 | 658 | 150,000 | 678 | 2 | PB + BSA 6 mg/mL | 685 | 701 | 34 |
2 Ethanol
3 Toluene
Frequently Used Fluorescence Excitation Sources
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| Dog spermatozoa stained with a mixture of K35 and K8-1400 fluorescent probes (450–490-nm band-pass excitation filter and 500-nm cutoff emission filter were used) | Dog spermatozoa stained with a mixture of K35 and K8-1400 fluorescent probes (545–580-nm band-pass excitation filter and 610–800-nm band-pass emission filter were used) |
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| Dog spermatozoa stained with K35 fluorescent probe (450–490-nm band-pass excitation filter and 500-nm cutoff emission filter were used) | Dog spermatozoa stained with a mixture of K8-1412 fluorescent probes (595–650-nm band-pass excitation filter and 670–740-nm band-pass emission filter were used) |