Fluorescence Imaging

Fluorescence imaging utilizes light produced by fluorescent species introduced into the specimen of interest. SETA BioMedicals has developed a series of proprietary probes and labels that are extremely bright and photostable fluorescent dyes for 2P, in vivo, and conventional imaging applications.

Peripheral blood samples stained with CD81 — Seta-APC-780

Mouse egg cell stained with Square-635 and Square-460

The group of Prof. Weissleder at the Harvard Medical School has synthesized, characterized, and tested three compatible imaging probes based on GLP1R targeting peptides to visualize pancreatic beta cells by intravital microscopy. Of the three conjugates (Alexa-488, Alexa-568, and SeTau-647) presented in their investigation, the performance of squaraine rotaxne dye SeTau 647 (K9-4148) was by far the best in this series. They determined that SeTau-647 had the highest signal-to-background ratio and the best in vivo imaging properties (2-photon as well as conventional)."Here we present the first generation of two-photon beta cell specific in vivo imaging probes based on GLP1R targeting peptides. Among the three compounds of potential interest, we found quite unexpectedly that a squarine-rotaxane conjugate (2PEx-SeTau-647) had near ideal in vivo imaging characteristics".

The same group also used a CD8 antibody labelled with SeTau-647-maleimide (K9-4148) in an intravital time-lapse microscopy (IVM) experiment [33].

			Excitation Light Sources												Characteristics						Characteristics
Product Number (Specs Sheet)	Product Name (Product Info)	Target Group	380	405	436	488	532	594	635	650	680	700	750	780	Medium	λ abs [nm]	ε [M ^–1 cm^–1]	λ em [nm]	QY [%]	FLT [ns]	Medium	λ abs [nm]	ε [M^–1 cm^–1]	λ em [nm]	QY [%]	FLT [ns]	Buy

K35 K35	K35 K35		•	•	•										Toluene	410	10,000	495	60		EtOH	430	13,200	552	0.01
K5-3222 K5-3222	Seta-580-NHS Seta-580-NHS	NH₂					•								PB 7.4	578	90,000	602	55		BSA, D/P=1	585		609	15
K6-207 K6-207	3-DAB 3-DAB			•	•	•									Toluene	445	9,700	582	43		EtOH	460	8,600	665	22	4.0
K6-208 K6-208	2-DAB 2-DAB			•	•	•									Toluene	464	3,600	551	31		EtOH	480	2,900	670	1.1
K7-1500 K7-1500	Q535 Q535		•	•	•	•	•	•							Toluene	542	15,000
K8-1342 K8-1342	Seta-670-NHS Seta-670-NHS	NH₂	•	•					•	•					PB 7.4	667	180,000	688	7	0.42	BSA, D/P=1.5	681	180,000	696	27	2.43
K8-1346 K8-1346	Seta-670-Azide Seta-670-Azide	triple-CC	•	•					•	•					PB 7.4	667	180,000	686	7		PB 7.4	667	180,000	686	7
K8-1350 K8-1350	Square-670-Carboxy Square-670-Carboxy		•	•					•	•					PB 7.4	656		676	1.7	0.29	6g/L BSA	673		693	29
K8-1351 K8-1351	Square-660-Carboxy Square-660-Carboxy		•	•					•	•					PB 7.4	657	182,000	676	3	0.27	2g/L BSA	679	182,000	696	45	3.56
K8-1355 K8-1355	Square-680-Carboxy Square-680-Carboxy		•	•					•	•	•				PB 7.4	654	138,000	670	1		1g/L BSA	688	168,000	708	62
K8-1357 K8-1357	Square-680-NHS Square-680-NHS	NH₂	•	•					•	•					MeOH	668	208,000	690	10		CHCl₃	683	202,000	701	47
K8-1384 K8-1384	Seta-700-NHS Seta-700-NHS	NH₂	•	•						•	•	•			PB 7.4	687	177,000	703	6		IgG D/P=1	705	177,000	715	4
K8-1407 K8-1407	Square-650-pH-NHS Square-650-pH-NHS	NH₂						•	•	•					PB 5.6	653	135,000	671	16	1.17	IgG, D/P=0.8 pH=2.0	662	135,000	677	7	1.52
K8-1440 K8-1440	Square-670 Square-670		•	•	•				•	•	•				pH 7.4	659		677	0.09		1 g/L BSA	683		702	50
K8-1500 K8-1500	Square-655 Square-655		•	•					•	•					PB 7.4	622	177,000	644	0.09		1 g/L BSA	652	188	672	80
K8-1641 K8-1641	Seta-632-Maleimide Seta-632-Maleimide	SH							•						PB 7.4	633	270,000	642	5
K8-1642 K8-1642	Seta-632-NHS Seta-632-NHS	NH₂							•						PB 7.4	632	280,000	641	6		IgG, D/P=1	637		647	18
K8-1663 K8-1663	Seta-633-NHS Seta-633-NHS	NH₂						•	•						PB 7.4	633	250,000	644	7	0.25	IgG D/P=1	637	250,000	647	26	1.49
K8-1672 K8-1672	Seta-646-NHS Seta-646-NHS	NH₂							•						PB 7.4	646	207,000	656	10	0.38	IgG, D/P=1	650	207,000	661	33
K8-1682 K8-1682	Seta-660-di-NHS Seta-660-di-NHS	NH₂							•	•					PB 7.4	661	220,000	672	11	0.9	IgG D/P=1	665	220,000	675	49
K8-1696 K8-1696	Seta-633-Azide Seta-633-Azide	triple-CC						•	•						PB 7.4	633	250,000	644	7		PB 7.4	633	250,000	644	7
K8-3335 K8-3335	Seta-555-NHS Seta-555-NHS	NH₂					•								PB 7.4	555	155,000	570	7		IgG, D/P=1	556		571	13
K8-3345 K8-3345	Seta-555-DBCO Seta-555-DBCO	N₃					•								PB 7.4	555	155,000	570	7
K8-3346 K8-3346	Seta-555-Azide Seta-555-Azide	triple-CC					•								PB 7.4	555	155,000	570	7
K8-5035 K8-5035	Seta-650-NHS Seta-650-NHS	NH₂							•	•					PB 7.4	651	200,000	671	28		IgG, D/P=1	654		673	37
K8-5036 K8-5036	Seta-650-Maleimide Seta-650-Maleimide	SH							•	•					PB 7.4	652	200,000	672	28
K8-5045 K8-5045	Seta-650-DBCO Seta-650-DBCO	N₃							•	•					PB 7.4	653	200,000	674	28
K8-5046 K8-5046	Seta-650-Azide Seta-650-Azide	triple-CC							•	•					PB 7.4	651	200,000	671	28
K8-7045 K8-7045	Seta-750-DBCO Seta-750-DBCO	N₃									•	•	•		PB 7.4	754	234,000	782	21
K8-7046 K8-7046	Seta-750-Azide Seta-750-Azide	triple-CC									•	•	•		PB 7.4	750	200,000	779	22
K8-7055 K8-7055	Seta-780-NHS Seta-780-NHS	NH₂										•	•	•	PB 7.4	777	215,000	807	1.2
K9-4142 K9-4142	SeTau-647-di-NHS SeTau-647-di-NHS	NH₂							•	•					PB 7.4	650	200,000	694	65	3.2	IgG, D/P=0.5	649	200,000	691	58	3.2
K9-4145 K9-4145	SeTau-633-Ethyl-Ester SeTau-633-Ethyl-Ester								•	•					CHCl₃	634	105,000	683	68
K9-4148 K9-4148	SeTau-647-Maleimide SeTau-647-Maleimide	SH							•	•					PB 7.4	648	200,000	692	45	3.2	PB 7.4	648	200,000	692	45	3.2
K9-4149 K9-4149	SeTau-647-NHS SeTau-647-NHS	NH₂							•	•					PB 7.4	649	200,000	695	61	3.2	IgG, D/P=1	648	200,000	694	59	3.3
K9-4150 K9-4150	SeTau-647 SeTau-647								•	•					PB 7.4	647	211,000	693	59	3.1	PB 7.4	647	211,000	693	59	3.1
K8-3402 K8-3402	Zy3-NHS Zy3-NHS	NH₂					•								PB 7.4	549	150,000	564	4	0.3
K8-3403 K8-3403	Zy3-Maleimide Zy3-Maleimide	SH					•								PB 7.4	549	150,000	564	4	0.3
K8-1388 K8-1388	Seta-700-di-NHS Seta-700-di-NHS	NH₂	•	•						•	•	•			PB 7.4	688	180,000	704	11		IgG D/P=1	696		713	23
K8-1341 K8-1341	Seta-670-Maleimide Seta-670-Maleimide	SH	•	•					•	•					PB 7.4	667	180,000	688	7
K8-1402 K8-1402	Square-650 Square-650								•	•					CHCl₃	654	210,000	671	53
K8-1671 K8-1671	Seta-646-Maleimide Seta-646-Maleimide	SH							•						PB 7.4	647	210,000	657	8		IgG, D/P=1	650		661	31
K8-3010 K8-3010	Square-460 Square-460		•	•	•	•									PB 7.4	467	50,000	515	0.9		6g/L BSA	469		512	8.6
K4-205 K4-205	Seta-375-NHS Seta-375-NHS	NH₂	•												PB 7.4	372	24,500	476	86	3.1	IgG, D/P=5	372	24,500	480	42	3.5
K7-548 K7-548	SeTau-405-Maleimide SeTau-405-Maleimide	SH	•	•											PB 7.4	405	13,800	518	51	9.1	EtOH	391	15,000	498	32	8.5
cl-APC cl-APC	Crosslinked APC Crosslinked APC							•	•	•					PB 7.4	650	700,000	662
K8-1405 K8-1405	Square-650-pH-Carboxy Square-650-pH-Carboxy	NH₂						•	•	•					PB 5.6	653	135,000	671	16	1.17	pH 9.0	535	48,000	663	9	0.53
K10-6801 K10-6801	Seta-PerCP-680 Seta-PerCP-680				•	•	•								PB 7.4	481	380,000	676
K10-7801 K10-7801	Seta-APC-780 Seta-APC-780								•	•					PB 7.4	651	1,100,000	780
K10-6701 K10-6701	Seta-R-PE-670 Seta-R-PE-670					•	•								PB 7.4	488	1,100,000	671
K8-7522 K8-7522	Seta-750-NHS Seta-750-NHS	NH₂									•	•	•		PB 7.4	753	230,000	780	14		IgG D/P=1	756		783	17
K10-0775 K10-0775	Seta-R-PE-775 Seta-R-PE-775					•	•								PB 7.4	488	1,100,000	774
K10-7051 K10-7051	Seta-APC-705 Seta-APC-705								•	•					PB 7.4	655	1,100,000	703
K4-225 K4-225	Seta-385-NHS Seta-385-NHS	NH₂	•												PB 7.4	384	25,000	512	78		IgG, D/P=6	387	25,000	505	29
K6-5052 K6-5052	Seta-405 NHS Seta-405 NHS	NH₂	•	•											PB 7.4	401	30,000	452	70		IgG D/P=1	405		453	56
K4-215 K4-215	Seta-400-NHS Seta-400-NHS	NH₂	•	•											PB 7.4	399	24,000	576	34		IgG D/P=1	401		569	19
K9-4179 K9-4179	SeTau-680-NHS SeTau-680-NHS	NH₂							•	•	•				PB 7.4	683	295,000	705	58	2.9	IgG D/P=1	682		703	47	3.1
K9-4154 K9-4154	SeTau-647-DBCO SeTau-647-DBCO	N₃							•	•					PB 7.4	649	200,000	690	60	3.2
K9-3152 K9-3152	SeTau-488-NHS SeTau-488-NHS	NH₂				•									PBS 7.4	486	59,000	532	27

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