Product Name: SQ-565-NHS

Product Number:
K8-1902
Target Group:
NH2
Excitation Light Sources:
Medium 1:
Water
λAb max. [nm]:
560
ε [M–1 cm–1]:
22000
QY [%]:
0
FLT [ns]:
0
Medium 2:
CHCl3
λAb max. [nm]:
565
Medium 3:
CHCl3
λAb max. [nm]:
565
Specs Sheet:
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Description

  • Amine-reactive, non-fluorescent quencher containing one reactive NHS-ester group
  • Very broad visible-wavelength absorption spectrum
  • Efficient energy transfer acceptor for green- to orange-fluorescent dyes (FAM, TET, JOE, TAM, ROX, Cy3, Cy3.5,

Alexa 532, Alexa 546, Alexa 568) in FRET applications

 

Applications

  • Covalent labeling of proteins, peptides, amino-modified DNA and amino-modified oligonucleotides
  • Energy Transfer (RET) acceptor for molecular beacons and other FRET based applications
  • Real-time PCR
  • Homogeneous Assays

 

Advantages

  • pH-insensitive between pH 3 and pH 10 
  • Good aqueous solubility; this label does not alter the solubility of the protein conjugate 
  • Low molecular weight  — SQ dyes do not add substantial mass to the conjugates 
  • Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides