Albumin Determination

		Excitation Light Sources												Characteristics						Characteristics
Product Number (Specs Sheet)	Product Name (Product Info)	380	405	436	488	532	594	635	650	680	700	750	780	Medium	λ abs [nm]	ε [M –1 cm–1]	λ em [nm]	QY [%]	FLT [ns]	Medium	λ abs [nm]	ε [M–1 cm–1]	λ em [nm]	QY [%]	FLT [ns]	Buy
K35 K35	K35 K35	•	•	•										Toluene	410	10,000	495	60		EtOH	430	13,200	552	0.01
K8-1355 K8-1355	Square-680-Carboxy Square-680-Carboxy	•	•					•	•	•				PB 7.4	654	138,000	670	1		1g/L BSA	688	168,000	708	62
K8-1500 K8-1500	Square-655 Square-655	•	•					•	•					PB 7.4	622	177,000	644	0.09		1 g/L BSA	652	188	672	80

K35 is a fluorescent probe for determination of albumin binding sites in plasma and serum [19, 20].

Square dyes exhibit high extinction coefficients and lower quantum yields in aqueous buffer but high quantum yields and long fluorescence lifetimes in presence of serum albumin. As an example Square-655 is a fluorescent probe with an extinction coefficient of 177 000 M^-1cm^-1 and a quantum yield of 80% in presence of albumin. These features make them the most sensitive fluorescent probes for the measurement of not only albumin but also other proteins. Because the binding constants of Square dyes to albumin are of the same order as for some of the common drugs these dyes are also useful for the estimation of drug-binding constants using the dye-displacement method.

Please contact us for more information.