Genomics

Genomics  is a branch of molecular biology relating to the structure, function, evolution, and mapping of genomes and more specifically relates to DNA, DNA sequencing methods and bioinformatics.

Below is a list of fluorescent labels (specifically maleimides) including Zy-dyes  that are commonly used for labeling of thiol-modified oligonucleotieds. They also include labels with long lifetimes for fluorescence lifetimes (FLT) and/or fluorescence polarization (FP) applications.

			Excitation Light Sources												Characteristics
Product Number (Specs Sheet)	Product Name (Product Info)	Target Group	380	405	436	488	532	594	635	650	680	700	750	780	Medium	λ abs [nm]	ε [M –1 cm–1]	λ em [nm]	QY [%]	FLT [ns]	Buy
K8-1641 K8-1641	Seta-632-Maleimide Seta-632-Maleimide	SH							•						PB 7.4	633	270,000	642	5
K8-5036 K8-5036	Seta-650-Maleimide Seta-650-Maleimide	SH							•	•					PB 7.4	652	200,000	672	28
K9-4148 K9-4148	SeTau-647-Maleimide SeTau-647-Maleimide	SH							•	•					PB 7.4	648	200,000	692	45	3.2
K8-3403 K8-3403	Zy3-Maleimide Zy3-Maleimide	SH					•								PB 7.4	549	150,000	564	4	0.3
K8-5403 K8-5403	Zy5-Maleimide Zy5-Maleimide	SH							•						PB 7.4	647	250,000	665	27	1
K8-5423 K8-5423	Zy5.5-Maleimide Zy5.5-Maleimide	SH							•	•	•				PB 7.4	676	190,000	694	23	1
K8-1341 K8-1341	Seta-670-Maleimide Seta-670-Maleimide	SH	•	•					•	•					PB 7.4	667	180,000	688	7
K8-1671 K8-1671	Seta-646-Maleimide Seta-646-Maleimide	SH							•						PB 7.4	647	210,000	657	8
K7-548 K7-548	SeTau-405-Maleimide SeTau-405-Maleimide	SH	•	•											PB 7.4	405	13,800	518	51	9.1
K8-2100 K8-2100	YOSeta-1 (1mM solution in DMSO) YOSeta-1 (1mM solution in DMSO)					•									dsDNA	492	98,800	509	52
K8-3003 K8-3003	Seta-470-Maleimide Seta-470-Maleimide	SH		•	•	•									Water	469	50,000	521	1.9

^new Staining of double-stranded DNA: YOSeta-1 is a cell-impermeant fluorescent dye used for staining of double-stranded DNA similar to YOYO-1. Unlike YOYO-1, YOSeta-1 is highly soluble in aqueous buffers. The free YOSeta-1 dye has a very low fluorescence quantum yield but the QY increases several thousand times upon binding to double-stranded DNA (see specs sheet). The probe is available as a 1 mM solution in DMSO.

Absorption and emission spectrum of YOSeta-1 in presence of dsDNA