Tandem Conjugates

Phycobiliprotein-tandem-conjugates permit the simultaneous detection of several parameters on cell surfaces using the 488 nm or 633 nm laser lines and other light sources for similar wavelengths. We refer you to Flow Cytometry for a list of common fluorescently labeled phycobiliproteins tandem dyes that are frequently used in Flow Cytometry, including a list of our superior Seta dye and Seta tandem dye replacements.

SETA BioMedicals has spent several years researching to optimize and produce these phycobiliprotein-tandems. These ready-made tandems of R-PE, PerCP, and APC are optimized for brightness, spillover, and photostability. Please refer to specs sheets below for more detailed information.

 

		Excitation Light Sources												Characteristics
Product Number (Specs Sheet)	Product Name (Product Info)	380	405	436	488	532	594	635	650	680	700	750	780	Medium	λ abs [nm]	ε [M –1 cm–1]	λ em [nm]	QY [%]	FLT [ns]	Buy
K10-6801 K10-6801	Seta-PerCP-680 Seta-PerCP-680			•	•	•								PB 7.4	481	380,000	676
K10-7801 K10-7801	Seta-APC-780 Seta-APC-780							•	•					PB 7.4	651	1,100,000	780
K10-6701 K10-6701	Seta-R-PE-670 Seta-R-PE-670				•	•								PB 7.4	488	1,100,000	671
K10-0775 K10-0775	Seta-R-PE-775 Seta-R-PE-775				•	•								PB 7.4	488	1,100,000	774
K10-7051 K10-7051	Seta-APC-705 Seta-APC-705							•	•					PB 7.4	655	1,100,000	703

 

The table below shows a listing of our proprietary, superior replacements for the most common tandems currently used in flow cytometry: 

 

Laser Source	Common Tandems Used	Superior Seta Replacements
488 nm
	R-PE-Cy5	Seta-R-PE-670
	PerCP-Cy5.5, R-PE-Cy5.5	Seta-PerCP-680
	R-PE-Cy7	Seta-R-PE-775
633 nm
	APC-AF700	Seta-APC-705
	APC-Cy7, APC-H7, APC-AF750	Seta-APC-780