Seta-750-DBCO (K8-7045) is a highly hydrophilic click chemistry reagent containing one dibenzocyclo-octyne (DBCO) group for strain-mediated cycloaddition reactions with azides. It features the same excitation and emission wavelengths as Cy7, Alexa Fluor™ 750, or Seta-750 and can therefore be used with these filter sets. It combines high photostability and brightness.
Applications
Strain-mediated click chemistry reactions with azide-modified reagents and biomolecules.
Advantages
The dye is excitable with NIR light sources and perfectly suited for excitation with 680-nm, 700-nm, and 750-nm LEDs and diode lasers
Sensitive; high extinction coefficients and high quantum yields (about twice as high as Alexa 750)
pH-insensitive between pH 3 and pH 10
Good aqueous solubility
High photostability; e.g. compared to fluorescein, Cy7 or Alexa 750
Low molecular weight — Seta-750 does not add substantial mass to the conjugates