In the absence of Vancomycin, the close proximity and efficient electron-transfer from Seta-640 (K8-1662) to anthraquinone causes significant fluorescence quenching of the dye (“off”-state). When Vancomycin is added, it binds with high affinity in a 2:1 ratio to the -d-Ala-d-Ala ligands, resulting in a 30 times increase in fluorescence intensity (“on”-state).
Excitation Light Sources | Characteristics | ||||||||||||||||||||
Product Number (Specs Sheet) |
Product Name (Product Info) |
Target Group | 380 | 405 | 436 | 488 | 532 | 594 | 635 | 650 | 680 | 700 | 750 | 780 | Medium | λ abs [nm] |
ε [M –1 cm–1] |
λ em [nm] |
QY [%] |
FLT [ns] |
Buy |
K8-1662 | Seta-640-di-NHS | NH2 | • | PB 7.4 | 636 | 181000 | 646 | 13 | 0.7 |