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Products
Reactive Labels
Square Labels
Seta Labels
SeTau Lifetime Labels
SQ Dyes (Reactive Quenchers)
Zy Dyes (Cy or Cyanine Dye Analogs)
Fluorescent Probes
Intensity Probes
Lifetime Probes
Quencher Probes
Fluorescence Standards
Fluorescence Lifetime Standards
Polarization Standards
Dyes for Particle Encoding
Squaraine Rotaxanes
Proteins & Conjugates
Phycobiliproteins
Tandem Dyes
Streptavidin Conjugates
Applications
Fluorescence Imaging
NIR Fluorescence Imaging
Flow Cytometry
DNA and Protein Microarrays
2-Photon Microscopy
Superresolution Microscopy
Single Molecule Applications
Immunofluorescence
Click Chemistry
Fluorescence Lifetime (FLT)
Fluorescence Lifetime Imaging
Fluorescence Polarization
FRET
Genomics
FCS
pH Sensing Applications
Plasmonics
Proteomics
GE/DIGE
Albumin Determination
Cholesterol & Triglycerides Measurement
Viscosity Measurements
Services
Custom Synthesis
Consulting
Resources
Software
Flyers
Fluorescence Excitation Sources
Procedures
Photo Gallery
Publications
Company
Partnerships
News
Announcements
Privacy Policy
Distributors of SETA BioMedicals
Contact Us
Terms and Conditions of Sale
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K4-215
Product Name: Seta-400-NHS
Product Number:
K4-215
Target Group:
NH
2
Excitation Light Sources:
Medium 1:
PB 7.4
λ
Ab
max. [nm]:
399
ε [M
–1
cm
–1
]:
24000
λ
Em
max. [nm]:
576
QY [%]:
34
Medium 2:
IgG D/P=1
λ
Ab
max. [nm]:
401
λ
Em
max. [nm]:
569
QY [%]:
19
Specs Sheet:
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Description
Positively charged, water-soluble dye with one NHS-ester group and an extremely large Stokes shift
Applications
Covalent labeling of proteins, DNA and oligonucleotides
Resonance Energy Transfer (RET)
Flow Cytometry
Immunofluorescence
Homogeneous Assays
Advantages
Perfectly suited for excitation with 370-nm, 380-nm and 405-nm diode lasers
Extremely large Stokes' shift
of over 170 nm
Low molecular weight —
Seta
dyes do not add substantial mass to the conjugates
Soluble in aqueous buffers
Ideal for non-radioactive labeling of proteins, DNA probes and oligonucleotides