Product Name: SeTau-405-NHS

Product Number:
K7-547
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
405
ε [M–1 cm–1]:
13800
λEm max. [nm]:
518
QY [%]:
80
FLT [ns]:
9.3
Medium 2:
IgG, D/P=8
λAb max. [nm]:
405
ε [M–1 cm–1]:
13800
λEm max. [nm]:
518
QY [%]:
19
FLT [ns]:
8.4
Medium 3:
BSA, D/P=3
λAb max. [nm]:
406
λEm max. [nm]:
518
FLT [ns]:
7.6
Medium 4:
EtOH
λAb max. [nm]:
391
ε [M–1 cm–1]:
15000
λEm max. [nm]:
498
QY [%]:
55
Specs Sheet:
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Description

  • Hydrophilic, amine-reactive, long-lifetime label containing one NHS-ester group, with one positive charge and chloride as the counter-ion.

 

Applications

  • Covalent labeling of proteins, amino-modified DNA, amino-modified oligonucleotides and lipids.
  • Fluorescence lifetime assays.
  • Fluorescence polarization-based assays of high molecular weight antigens.

 

Advantages

  • Highly fluorescent label for proteins and oligos.
  • High quantum yield (Q.Y.) ~ 80 % (water)
  • Long fluorescence lifetime of 9 ns in water
  • Perfectly suited for excitation with the 380-nm and 404-nm diode lasers
  • Large Stokes' shift of over 100 nm
  • High fundamental polarization R0 = 475 mP
  • Highly soluble in aqueous buffer (500 mg/L at 20°C)