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K7-548
Product Name: SeTau-405-Maleimide
Product Number:
K7-548
Target Group:
SH
Excitation Light Sources:
Medium 1:
PB 7.4
λ
Ab
max. [nm]:
405
ε [M
–1
cm
–1
]:
13800
λ
Em
max. [nm]:
518
QY [%]:
51
FLT [ns]:
9.1
Medium 2:
EtOH
λ
Ab
max. [nm]:
391
ε [M
–1
cm
–1
]:
15000
λ
Em
max. [nm]:
498
QY [%]:
32
FLT [ns]:
8.5
Specs Sheet:
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Description
Hydrophilic, thiol-reactive, long-lifetime label containing one maleimide group, with one positive charge and chloride as the counter-ion.
Applications
Covalent labeling of proteins, thiol-modified DNA, thiol-modified oligonucleotides and lipids.
Fluorescence lifetime assays.
Fluorescence polarization-based assays of high molecular weight antigens.
Advantages
Highly fluorescent label for proteins and oligos.
High quantum yield (Q.Y.) ~ 51 % (water).
Long fluorescence lifetime of 9 ns in water.
Perfectly suited for excitation with the 380-nm and 404-nm diode lasers .
Large Stokes' shift of over 100 nm.
High fundamental polarization
P
0
= 475 mP.
Highly soluble in aqueous buffer (500 mg/L at 20°C).