Product Name: Seta-670-Azide
Product Number:
K8-1346 Target Group:
triple-CCExcitation Light Sources:
Medium 1:
PB 7.4λAb max. [nm]:
667ε [M–1 cm–1]:
180000λEm max. [nm]:
686QY [%]:
7Medium 2:
PB 7.4λAb max. [nm]:
667ε [M–1 cm–1]:
180000λEm max. [nm]:
686QY [%]:
7Specs Sheet:
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Description
- Highly hydrophilic, alkyne-reactive reagent for click chemistry containing one azide function. Azides react with C≡C triple bonds in either a Cu(I)-catalyzed or Cu-free 1,3-dipolar cycloaddition reaction to a triazole.
Applications
- Click Chemistry reagent
- Fluorescence intensity and fluorescence polarization-based applications
- Fluorescence Resonance Energy Transfer (FRET) applications
- Single Molecule Applications – Seta-670 shows extreme low blinking in single molecule measurements
Advantages
- Perfectly suited for excitation with the 380, 404, 635, 670-nm diode lasers, LEDs, and UV light
- Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules
- Quantum yield is highly increased after covalent and non-covalent association with proteins
- pH-insensitive between pH 3 and pH 10
- Good aqueous solubility: this label does not alter the solubility of the bioconjugate
- Photostability: Higher photostability as compared to Alexa Fluor™ 647 or Cy5TM
- Low molecular weight: Seta dyes do not add substantial mass to the conjugates
- Ideal for non-radioactive labeling of alkyne-modified proteins, DNA and oligonucleotides
