Product Name: Square-660-NHS

Product Number:

K8-1352

Target Group:

NH₂

Excitation Light Sources:

Medium 1:

PB 7.4

λ_Ab max. [nm]:

657

ε [M^–1cm^–1]:

182000

λ_Em max. [nm]:

676

QY [%]:

FLT [ns]:

0.27

Medium 2:

BSA D/P=1.2

λ_Ab max. [nm]:

676

ε [M^–1cm^–1]:

182000

λ_Em max. [nm]:

695

QY [%]:

FLT [ns]:

3.32

Specs Sheet:

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Description

Amine-reactive, lifetime-sensitive fluorescent label containing one reactive NHS-ester group

Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
Fluorescence polarization assays
Homogeneous lifetime-based assays
FRET based applications
Flow Cytometry

Perfectly suited for excitation with the 670-nm diode laser, 404-nm diode laser, and UV light
Sensitive; high extinction coefficients and high quantum yields up to 25% after covalent attachment to proteins and amino-modified oligonucleotides.
Low non-specific binding
Lifetime sensitive label: fluorescence lifetime will change 10-fold upon binding to an oligonucleotide
pH-insensitive between pH 3 and pH 10
High photostability; e.g. compared to fluorescein or Cy5^TM
Low molecular weight — Square dyes do not add substantial mass to the conjugates
Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides