Hydrophobic, amine-reactive fluorescent label containing one reactive NHS-ester group.
Applications
Covalent labeling of amino-modified lipids and oligonucleotides
Fluorescence Resonance Energy Transfer (FRET) applications
Homogeneous Assays
Advantages
The dye is excitable with both UV-blue and red-NIR light sources and is perfectly suited for excitation with 380-nm, 404-nm, 635-nm, and 670-nm LEDs and diode lasers
Sensitive; high extinction coefficients and quantum yields of protein conjugates
Quantum yields increase upon covalent association with biomolecules
pH-insensitive between pH 3 and pH 10
High photostability, e.g., compared to fluorescein or Cy5TM
Low molecular weight — Square dyes do not add substantial mass to the conjugates
Ideal for non-radioactive labeling of amino-modified lipids