Product Name: Seta-670-pH-di-NHS

Product Number:
K8-1367
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 6.0
λAb max. [nm]:
672
ε [M–1 cm–1]:
93000
λEm max. [nm]:
694
QY [%]:
19
Medium 2:
IgG, D/P=1
λAb max. [nm]:
684
λEm max. [nm]:
703
QY [%]:
7
Specs Sheet:
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Description

  • Amine-reactive, pH-sensitive fluorescent label containing two reactive NHS-ester groups and pKa in the physiological pH range.

 

Applications

  • pH-sensing applications.
  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides.
  • Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule.
  • Resonance Energy Transfer (RET).
  • Immunofluorescence.
  • Homogeneous Assays.
  • Assessment of protein structure.

 

Advantages

  • Perfectly suited for excitation with the 670-nm, 650-nm, 635-nm, 404-nm, and 370-nm diode lasers and LEDs.
  • Sensitive; high extinction coefficients and high quantum yields up to 20% after covalent attachment to proteins. 
  • Low non-specific binding.
  • pH-sensitive between pH 7 and pH 11, and pH-insensitive between pH 3 and pH 7.
  • Good aqueous solubility; this label does not alter the solubility of the protein conjugate. 
  • Low molecular weight  — Seta dyes do not add substantial mass to the conjugates. 
  • Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides.