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K8-1384
Product Name: Seta-700-NHS
Product Number:
K8-1384
Target Group:
NH
2
Excitation Light Sources:
Medium 1:
PB 7.4
λ
Ab
max. [nm]:
687
ε [M
–1
cm
–1
]:
177000
λ
Em
max. [nm]:
703
QY [%]:
6
Medium 2:
IgG D/P=1
λ
Ab
max. [nm]:
705
ε [M
–1
cm
–1
]:
177000
λ
Em
max. [nm]:
715
QY [%]:
4
Medium 3:
MeOH
λ
Ab
max. [nm]:
701
ε [M
–1
cm
–1
]:
175000
λ
Em
max. [nm]:
719
QY [%]:
18
Specs Sheet:
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Description
Water soluble, amine-reactive fluorescent label containing one reactive NHS-ester group.
Applications
Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule
Resonance Energy Transfer (RET)
Flow Cytometry
Immunofluorescence
Gene Expression
Homogeneous Assays
Assessment of protein structure
Advantages
Perfectly suited for excitation with the 380, 405, 650, 680, and 700-nm diode lasers, and UV light
pH-insensitive between pH 4 and pH 9
Good aqueous solubility; this label does not alter the solubility of the protein conjugate
High photostability; e.g. compared to fluorescein or
Cy5
TM
and
Cy7
TM
Low molecular weight —
Seta
dyes do not add substantial mass to the conjugates
Ideal for non-radioactive labeling of proteins, amino-modified probes and amino-modified oligonucleotides