Product Name: Seta-700-NHS

Product Number:

K8-1384

Target Group:

NH₂

Excitation Light Sources:

Medium 1:

PB 7.4

λ_Ab max. [nm]:

687

ε [M^–1cm^–1]:

177000

λ_Em max. [nm]:

703

QY [%]:

Medium 2:

IgG D/P=1

λ_Ab max. [nm]:

705

ε [M^–1cm^–1]:

177000

λ_Em max. [nm]:

715

QY [%]:

Medium 3:

MeOH

λ_Ab max. [nm]:

701

ε [M^–1cm^–1]:

175000

λ_Em max. [nm]:

719

QY [%]:

Specs Sheet:

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Description

Water soluble, amine-reactive fluorescent label containing one reactive NHS-ester group.

Covalent labeling of proteins, amino-modified DNA, and amino-modified oligonucleotides
Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule
Resonance Energy Transfer (RET)
Flow Cytometry
Immunofluorescence
Gene Expression
Homogeneous Assays
Assessment of protein structure

The dye is excitable with both UV-blue and red-NIR light sources and perfectly suited for excitation with 380-nm, 404-nm, 650-nm, 680-nm, and 700-nm LEDs and diode lasers
pH-insensitive between pH 4 and pH 9
Good aqueous solubility; this label does not alter the solubility of the protein conjugate
High photostability, e.g., compared to fluorescein or Cy5^TM and Cy7^TM
Low molecular weight — Seta dyes do not add substantial mass to the conjugates
Ideal for non-radioactive labeling of proteins, amino-modified probes, and amino-modified oligonucleotides