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Product Name: Square-650-pH-NHS

Product Number:
K8-1407
Target Group:
NH2
Excitation Light Sources:
Medium 1:
PB 5.6
λAb max. [nm]:
653
ε [M–1 cm–1]:
135000
λEm max. [nm]:
671
QY [%]:
16
FLT [ns]:
1.17
Medium 2:
IgG, D/P=0.8
pH=2.0
λAb max. [nm]:
662
ε [M–1 cm–1]:
135000
λEm max. [nm]:
677
QY [%]:
7
FLT [ns]:
1.52
Specs Sheet:
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Description

  • pH-sensitive, fluorescent label containing one reactive NHS-ester group and pKa in the physiological pH range (pKa = 7.1 - free dye, pKa ~ 6.3 when labeled to antibody).

 

Applications

  • Cell-based imaging applications (ratiometric or FLIM) of e.g. receptor translocations, plasma membrane associated receptor activation or GPCR-ligand interactions via endocytosis.
  • Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides and amino-modified lipids.

 

Advantages

  • Perfectly suited for excitation with the 594, 635 and 650-nm diode lasers.
  • Sensitive; high extinction coefficients and high quantum yields up to 16% after covalent attachment to biomolecules.
  • pH-label that exhibits intensity as well as lifetime-based changes with pH.
  • Good aqueous solubility; this label does not alter the solubility of the conjugate.
  • Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides.