Product Name: Seta-632-NHS

Product Number:

K8-1642

Target Group:

NH₂

Excitation Light Sources:

Medium 1:

PB 7.4

λ_Ab max. [nm]:

632

ε [M^–1cm^–1]:

280000

λ_Em max. [nm]:

641

QY [%]:

Medium 2:

IgG, D/P=1

λ_Ab max. [nm]:

637

λ_Em max. [nm]:

647

QY [%]:

Specs Sheet:

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Description

Covalent labeling of proteins, amino-modified DNA and amino-modified oligonucleotides
Fluorescence intensity and fluorescence polarization-based applications
Resonance Energy Transfer (RET)
Flow Cytometry
Immunofluorescence
Gene Expression
Homogeneous Assays
Assessment of protein structure

Perfectly suited for excitation with the 635 nm diode lasers
Sensitive; high extinction coefficients and high quantum yields after covalent attachment to biomolecules
Quantum yield is highly increased after covalent and non-covalent association with proteins
pH-insensitive between pH 3 and pH 10
Good aqueous solubility; this label does not alter the solubility of the bioconjugate
High photostability; e.g. compared to fluorescein or Cy5^TM
Low molecular weight — Seta dyes do not add substantial mass to the conjugates
Ideal for non-radioactive labeling of proteins, amino-modified oligonucleotides and amino-modified lipids