Product Name: Seta-635-pH-di-NHS

Product Number:

K8-1667

Target Group:

NH₂

Excitation Light Sources:

Medium 1:

PB 7.4

λ_Ab max. [nm]:

640

ε [M^–1cm^–1]:

188000

λ_Em max. [nm]:

656

QY [%]:

FLT [ns]:

1.8

Medium 2:

IgG, D/P=1

λ_Ab max. [nm]:

646

λ_Em max. [nm]:

663

QY [%]:

Specs Sheet:

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Description

Amine-reactive, pH-sensitive fluorescent label containing two reactive NHS-ester groups and pKa = 10.2 (free dye).

Covalent labeling of proteins, amino-modified DNA, amino-modified oligonucleotides and other small molecules.
Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule.

Perfectly suited for excitation with the 635-nm diode laser
Sensitive; high extinction coefficients and high quantum yields up to 30% after covalent attachment to proteins
Low non-specific binding
Good aqueous solubility; this label does not alter the solubility of the protein conjugate
High photostability
Low molecular weight — Seta dyes do not add substantial mass to the conjugates
Ideal for non-radioactive labeling of proteins, amino-modified DNA probes, amino-modified oligonucleotides and other small molecules