Product Name: Seta-635-pH-di-NHS
Product Number:
K8-1667 Target Group:
NH2Excitation Light Sources:
Medium 1:
PB 7.4λAb max. [nm]:
640ε [M–1 cm–1]:
188000λEm max. [nm]:
656QY [%]:
33FLT [ns]:
1.8Medium 2:
IgG, D/P=1λAb max. [nm]:
646λEm max. [nm]:
663QY [%]:
21Specs Sheet:
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Description
- Amine-reactive, pH-sensitive fluorescent label containing two reactive NHS-ester groups and pKa = 10.2 (free dye).
Applications
- Covalent labeling of proteins, amino-modified DNA, amino-modified oligonucleotides and other small molecules.
- Fluorescence Lifetime Label — this label exhibits a distinct lifetime change upon binding to a biomolecule.
Advantages
- Perfectly suited for excitation with the 635-nm diode laser
- Sensitive; high extinction coefficients and high quantum yields up to 30% after covalent attachment to proteins
- Low non-specific binding
- Good aqueous solubility; this label does not alter the solubility of the protein conjugate
- High photostability
- Low molecular weight — Seta dyes do not add substantial mass to the conjugates
- Ideal for non-radioactive labeling of proteins, amino-modified DNA probes, amino-modified oligonucleotides and other small molecules
