Product Name: SeTau-647-di-NHS
Product Number:
K9-4142 Target Group:
NH2Excitation Light Sources:
Medium 1:
PB 7.4λAb max. [nm]:
650ε [M–1 cm–1]:
200000λEm max. [nm]:
694QY [%]:
65FLT [ns]:
3.2Medium 2:
IgG, D/P=0.5λAb max. [nm]:
649ε [M–1 cm–1]:
200000λEm max. [nm]:
691QY [%]:
58FLT [ns]:
3.2Specs Sheet:
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Description
- Water-soluble, amine-reactive label containing two NHS-ester groups — brightest fluorescent label currently available for the 647-nm Kr-ion laser line
Applications
- Fluorescence imaging
- Fluorescence lifetime applications and fluorescence lifetime imaging (FLIM)
- Superresolution microscopy
- Immunofluorescence and FRET applications
- 2-Photon microscopy
- Single molecule applications
- Fluorescence correlation spectroscopy (FCS)
- Proteomics
Advantages
- Perfectly suited for excitation with the 635-nm, 647-nm, or 650-nm lasers
- Brightest dye currently available on the market for the red laser lines
- Highly sensitive: high extinction coefficient of 200,000 M–1cm–1 and high quantum yield of ~60% in aqueous environments
- Good aqueous solubility: this label does not alter the solubility of the protein conjugate
- Ozone stability: Higher ozone stability than Alexa Fluor™ 647 or Cy5™ enables array experiments to be performed with SeTau 647 under any environmental condition
- Large Stokes' shift: SeTau647 has a 44 nm Stokes' shift
- Extremely photostable: Much higher photostability as compared to Alexa Fluor™ 647, DyLight 650 , ATTO 647N or Cy5™ (see below)
- Fluorescence lifetime three times longer than that of Cy5™ or Alexa Fluor™ 647: 3.1 ns in water
- Ideal for non-radioactive labeling of proteins, amino-modified DNA probes and amino-modified oligonucleotides
