Product Name: SeTau-647-Maleimide

Product Number:
K9-4148
Target Group:
SH
Excitation Light Sources:
Medium 1:
PB 7.4
λAb max. [nm]:
648
ε [M–1 cm–1]:
200000
λEm max. [nm]:
692
QY [%]:
45
FLT [ns]:
3.2
Medium 2:
PB 7.4
λAb max. [nm]:
648
ε [M–1 cm–1]:
200000
λEm max. [nm]:
692
QY [%]:
45
FLT [ns]:
3.2
Specs Sheet:
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Description

  • Extremely bright, water-soluble, thiol-reactive label containing one maleimide group. The ideal label for proteins and other thiol-modified biomolecules including oligonucleotides.

 

Advantages

  • Perfectly suited for excitation with 635, 640, and 650-nm diode lasers.
  • Low quenching tendency at high dye-to-protein ratios compared to other labels e.g. Cy5.
  • Large Stokes’shiftof ~44 nm (about twice that of Cy5 or Alexa 647).
  • Considerably higher photostabilitycompared to fluorescein or other cyanine dyes (Cy5, Alexa or ATTO dyes).
  • High chemical stability against oxidation with peroxides or other oxygen species.
  • Several times longer fluorescence lifetime (t ~ 3 ns)compared to Cy5 or Alexa 647 (t ~ 1 ns).
  • Extremely bright label: most sensitive organic fluorescent label for proteins currently on the market for the 647-nm Kr-ion laser line.