Extremely bright, water-soluble, amine-reactive label containing one NHS-ester group. The ideal label for proteins and other amino-modified biomolecules including oligonucleotides.
Advantages
Perfectly suited for excitation with 640, 650, and 670-nm diode lasers
Low quenching tendency at high dye-to-protein ratios compared to other labels e.g. Cy5.5
Stokes’shift of ~31 nm (larger than for Cy5.5 or Alexa 660).
Considerably higher photostability compared to fluorescein or cyanine dyes (e.g. Cy5 or Cy5.5)
High chemical stability against oxidation with peroxides or other oxygen species
Longer fluorescence lifetime compared to Cy5.5 (2 ns) or Alexa 660 (0.37 ns) or Alexa 680 (0.36 ns)
Extremely bright label: most sensitive organic fluorescent label for proteins currently on the market for the 647-nm Kr-ion and 670 laser lines