Product Name: SeTau-680-NHS

Product Number:

K9-4179

Target Group:

NH₂

Excitation Light Sources:

Medium 1:

PB 7.4

λ_Ab max. [nm]:

683

ε [M^–1cm^–1]:

295000

λ_Em max. [nm]:

705

QY [%]:

FLT [ns]:

2.9

Medium 2:

IgG D/P=1

λ_Ab max. [nm]:

682

λ_Em max. [nm]:

703

QY [%]:

FLT [ns]:

3.1

Specs Sheet:

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Description

Extremely bright, water-soluble, amine-reactive squaraine-rotaxane label containing one NHS-ester group. The ideal label for proteins and other amino-modified biomolecules, including oligonucleotides.

Advantages

Perfectly suited for excitation with the 635-nm, 650-nm, 670-nm, and 680-nm LEDs and diode lasers
Low quenching tendency at high dye-to-protein ratios compared to other labels, e.g., Cy5.5™
Considerably higher photostability compared to other cyanine dyes (Cy5.5 or Alexa 680)
High chemical stability against oxidation with peroxides or other oxygen species
Longer fluorescence lifetime (τ ~ 3 ns) compared to Cy5.5 (τ ~ 1 ns) or Alexa 680 (τ ~ 1.2 ns)
Extremely bright label: SeTau 680: (Φ = 0.58, ε = 295,000 M^-1cm^-1 and Φ∙ε = 171,100 M^-1cm^-1) vs Alexa 680 (Φ = 0.36, ε = 183,000 M^-1cm^-1 and Φ∙ε = 65,880 M^-1cm^-1)