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The Seta dyes series spans the whole visible range and the NIR.
Seta-375-NHS (K4-205) is an extremely bright, reactive label for use with the UV 375 - 380 nm diode lasers. It is much brighter alternative for Alexa 350 with a much higher extiction coefficient and quantum efficiency. Exc./Em. max: 372/480 nm.
SeTau-405-NHS (K7-547) is a dye that is well suited for excitation with the blue 404 nm diode laser. Because of its large Stokes’ shift of more than 110 nm, the quenching tendency upon labeling to proteins is very low. Importantly the fluorescence lifetime of this dye is in the order of 9 ns, making it also a valuable probe for studying protein hydrodynamics (fluorescence polarization measurements). It is a good alternative for Pacific Orange to be used with Pacific Blue or V450 in flow cytometry applications. Compatible with Fluorescein emission filter. Exc./Em. max: 405/518 nm.
Seta-470-NHS (K8-3002) is a fluorescent label perfectly suited for the 447, 457 and 473 nm diode lasers. Its extinction coefficient is 50,000 M–1cm–1and it exhibits highly increased Q.Y.s after covalent attachment to proteins. Exc./Em. max: 469/515 nm.
Seta-555-NHS (K8-3335) is a direct replacement for Cy3, Alexa 555 or DyLight 550 and can therefore be used with these filter sets. Its extinction coefficient is 154,000 M–1cm–1and the Q.Y. of Seta-555 is about twice as high as that of Cy3.
Seta-632-NHS (K8-1642) is a better alternative to Alexa 633: firstly its absorption maximum is at 632 nm while that of Alexa 633 is blue-shifted to about 621 nm. Secondly Seta-632 has an extinction coefficient of 280,000 M–1cm–1as compared to 159,000 M–1cm–1 for Alexa 633. Emitting bright red fluorescence this dye can be measured with the Alexa 633 filter set. Exc./Em. max of IgG-conjugates: 637/647 nm.
Seta-633-NHS (K8-1663) is a hydrophilic, mono-reactive label for proteins and other biomolecules. It is extremely bright and has a low tendency for self-quenching even at higher D/P ratios. The extinction coefficient is 250,000 M–1cm–1as compared to 159,000 M–1cm–1 for Alexa 633 or 170,000 M-1cm-1 for DyLight 633, and quantum yields as high as 70% were measured with conjugates of this long-wavelength dye. It is optimally suited to be excited with the 633 nm spectral line of the He-Ne laser or the 635 nm line of red diode lasers and replaces Alexa 633 and/or DyLight 633. Exc./Em. max of IgG-conjugates: 637/647 nm.
Seta-646-NHS (K8-1672) is extremely bright (extinction coefficient is ~210,000 M–1cm–1, and the quantum yield of labeled antibodies are as high as 33% at a D/P ~ 1) and an ideal replacement of Cy5, Alexa 647 or DyLight 650 producing intense red fluorescence emission. It is also extremely photostable and can be optimally excited with the 647 nm spectral line of the Krypton-Argon laser. Exc./Em. max for conjugates: 650/661 nm.
Seta-660-di-NHS (K8-1682) is much brighter alterntive to Alexa 660: Its extinction coefficient is 220,000 M–1cm–1 as compared to 132,000 M–1cm–1 for Alexa 660, and quantum yields as high as 50% were measured from IgG-conjugates with this long-wavelength marker. It emits intense fluorescence in the far-red region of the light spectrum. Ex./Em. max: 661/672 nm.
Seta-680-NHS (K8-5075) is a dye that replaces Cy5.5, Alexa 680 or DyLight 680. The dye has an extinction coefficient of 200,000 M–1cm–1 as compared to 160,000 M-1cm-1 for DyLight 680 and its IgG-conjugates are bright and photostable. Ex./Em. max: 678/698 nm.
Seta-700-NHS (K8-1384) is an extremly bright, water-soluble fluorescent label with extremly high Q.Y.s of up to 0.44 when labeled to IgG. Replaces Alexa 700 (Q.Y. = 0.25). Ex./Em. max (IgG-Conjugate): 704/718 nm.
Seta-750-NHS (K8-7035) is a hydrophilic, mono-reactive label with almost identical absorption and emission as Cy7 or Alexa 750 and can therefore be used with these filter sets. It combines high photostability and brightness. Replacement for Cy7, Alexa 750 and DyLight 750. Its extinction coefficient is 235,000 M–1cm–1. Ex./Em. max: 750/779 nm.
All dyes are available in the following formats: carboxyl, NHS-esters and maleimides.
For additional product information we refer you to the specs sheets and the table below.
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