Fluorescent Probes
SETA BioMedicals offers probes for various types of applications:
Some of these specific probes are targeted to measure changes in lipid bilayer hydration of cells, cholesterol and triglycerides content in biological fluids and live/necrotic cell populations. We also offer probes for protein analysis. With quantum yields of 80% and extination cefficients in the order of 180,000 - 210,000 M-1cm-1. Square probes are on of the most sensitive dyes for the quantitative analysis of proteins.
K35 is a hydrophobic fluorescent probe for albumin binding sites in plasma and serum.
YO-Seta-1 (same as YOYO-1) is a high affinity cell-impermeable probe for staining of dsDNA.
Seta-Green is a high affinity, cell-impermeant nucleic acid stain for qPCR similar to SYBR Green-1.
Square-460 is an highly viscosity-sensitive fluorescent probe.
Our fluorescent rotaxane probes SeTau-647 and SeTau-633 exhibit extremely high chemical and photostability and are well suited for cellular imaging. They also have extremly high 2-photon action cross sections in the order of several thousand GM.
The pH-sensitive probe Square-650-pH is the only probe of its kind which shows lifetime sensitive responses in the physiological pH range. It is well suited for measurements of internal conversion and endocytic events.
| Excitation Light Sources | Characteristics | Characteristics | ||||||||||||||||||||||||
| Product Number (Specs Sheet) |
Product Name (Product Info) |
380 | 405 | 436 | 488 | 532 | 594 | 635 | 650 | 680 | 700 | 750 | 780 | Medium | λ abs [nm] |
ε [M –1 cm–1] |
λ em [nm] |
QY [%] |
FLT [ns] |
Medium | λ abs [nm] |
ε [M–1 cm–1] |
λ em [nm] |
QY [%] |
FLT [ns] |
Buy |
| K35 | K35 | • | • | • | Toluene | 410 | 10,000 | 495 | 60 | EtOH | 430 | 13,200 | 552 | 0.01 | ||||||||||||
| K6-207 | 3-DAB | • | • | • | Toluene | 445 | 9,700 | 582 | 43 | EtOH | 460 | 8,600 | 665 | 22 | 4.0 | |||||||||||
| K6-208 | 2-DAB | • | • | • | Toluene | 464 | 3,600 | 551 | 31 | EtOH | 480 | 2,900 | 670 | 1.1 | ||||||||||||
| K8-1350 | Square-670-Carboxy | • | • | • | • | PB 7.4 | 656 | 676 | 1.7 | 0.29 | 6g/L BSA | 673 | 693 | 29 | ||||||||||||
| K8-1351 | Square-660-Carboxy | • | • | • | • | PB 7.4 | 657 | 182,000 | 676 | 3 | 0.27 | 2g/L BSA | 679 | 182,000 | 696 | 45 | 3.56 | |||||||||
| K8-1355 | Square-680-Carboxy | • | • | • | • | • | PB 7.4 | 654 | 138,000 | 670 | 1 | 1g/L BSA | 688 | 168,000 | 708 | 62 | ||||||||||
| K8-1440 | Square-670 | • | • | • | • | • | • | pH 7.4 | 659 | 677 | 0.09 | 1 g/L BSA | 683 | 702 | 50 | |||||||||||
| K8-1443 | Square-730 | • | • | • | • | • | • | CHCl3 | 728 | 166,000 | 757 | 46 | 1g/L BSA | 722 | 745 | |||||||||||
| K8-1500 | Square-655 | • | • | • | • | PB 7.4 | 622 | 177,000 | 644 | 0.09 | 1 g/L BSA | 652 | 188 | 672 | 80 | |||||||||||
| K9-4145 | SeTau-633-Ethyl-Ester | • | • | CHCl3 | 634 | 105,000 | 683 | 68 | ||||||||||||||||||
| K9-4150 | SeTau-647 | • | • | PB 7.4 | 647 | 211,000 | 693 | 59 | 3.1 | PB 7.4 | 647 | 211,000 | 693 | 59 | 3.1 | |||||||||||
| K8-1402 | Square-650 | • | • | CHCl3 | 654 | 210,000 | 671 | 53 | ||||||||||||||||||
| K8-3010 | Square-460 | • | • | • | • | PB 7.4 | 467 | 50,000 | 515 | 0.9 | 6g/L BSA | 469 | 512 | 8.6 | ||||||||||||
| K8-1405 | Square-650-pH-Carboxy | • | • | • | PB 5.6 | 653 | 135,000 | 671 | 16 | 1.17 | pH 9.0 | 535 | 48,000 | 663 | 9 | 0.53 | ||||||||||
| K8-1610 | Square-635-Carboxy | • | • | pH 7.4 | 634 | 180,000 | 646 | 9 | pH 12.0 | 520 | - | - | - | |||||||||||||
| K8-1665 | Seta-635-pH-di-Carboxy | • | PB 7.4 | 640 | 188,000 | 656 | 33 | 1.8 | pH 12.0 | 519 | - | - | - | |||||||||||||
| K8-1365 | Seta-670-pH-di-Carboxy | • | • | • | • | • | PB 6.0 | 672 | 93,000 | 694 | 19 | pH 11.5 | 535 | - | - | - | ||||||||||
| K6-1037 | K37 | • | • | Toluene | 434 | 29,500 | 540 | 49 | Cholesterol in water | 540 | ||||||||||||||||
| K8-2100 | YOSeta-1 (1mM solution in DMSO) | • | dsDNA | 492 | 98,800 | 509 | 52 | |||||||||||||||||||
