Flow Cytometry

Flow Cytometry is a powerful technique to measure multiple parameters from cell populations. Flow cytometers differentiate cells based on their size, internal complexity, and fluorescence signature. Qualitative and quantitative analyses of cell populations have relevance for clinical and research applications.

Excitation Light Sources Characteristics
Product Number
(Specs Sheet)
Product Name
(Product Info)
Target Group 380 405 436 488 532 594 635 650 680 700 750 780 Medium λ abs
[nm]
ε
[M –1
cm–1]
λ em
[nm]
QY
[%]
FLT
[ns]
Buy
                   
K7-547
K7-547
SeTau-405-NHS
SeTau-405-NHS
NH2 PB 7.4 405 13,800 518 80 9.3
K7-545
K7-545
SeTau-425-NHS
SeTau-425-NHS
NH2 PB 7.4 425 4,200 545 39 26.2
K8-1384
K8-1384
Seta-700-NHS
Seta-700-NHS
NH2 PB 7.4 687 177,000 703 6
K8-1682
K8-1682
Seta-660-di-NHS
Seta-660-di-NHS
NH2 PB 7.4 661 220,000 672 11 0.9
K8-5035
K8-5035
Seta-650-NHS
Seta-650-NHS
NH2 PB 7.4 651 200,000 671 28
K8-5036
K8-5036
Seta-650-Maleimide
Seta-650-Maleimide
SH PB 7.4 652 200,000 672 28
K8-7055
K8-7055
Seta-780-NHS
Seta-780-NHS
NH2 PB 7.4 777 215,000 807 1.2
K9-4119
K9-4119
SeTau-665-NHS
SeTau-665-NHS
NH2 PB 7.4 664 160,000 712 53 3.1
K9-4149
K9-4149
SeTau-647-NHS
SeTau-647-NHS
NH2 PB 7.4 649 200,000 695 61 3.2
K8-1388
K8-1388
Seta-700-di-NHS
Seta-700-di-NHS
NH2 PB 7.4 688 180,000 704 11
K8-1341
K8-1341
Seta-670-Maleimide
Seta-670-Maleimide
SH PB 7.4 667 180,000 688 7
K4-205
K4-205
Seta-375-NHS
Seta-375-NHS
NH2 PB 7.4 372 24,500 476 86 3.1
K7-548
K7-548
SeTau-405-Maleimide
SeTau-405-Maleimide
SH PB 7.4 405 13,800 518 51 9.1
cl-APC
cl-APC
Crosslinked APC
Crosslinked APC
PB 7.4 650 700,000 662
R-PE
R-PE
Lyophilized R-PE
Lyophilized R-PE
PB 7.4 565 1,960,000 573
K10-6801
K10-6801
Seta-PerCP-680
Seta-PerCP-680
PB 7.4 481 380,000 676
K10-7801
K10-7801
Seta-APC-780
Seta-APC-780
PB 7.4 651 1,100,000 780
K10-6701
K10-6701
Seta-R-PE-670
Seta-R-PE-670
PB 7.4 488 1,100,000 671
K10-0775
K10-0775
Seta-R-PE-775
Seta-R-PE-775
PB 7.4 488 1,100,000 774
K10-7051
K10-7051
Seta-APC-705
Seta-APC-705
PB 7.4 655 1,100,000 703
K7-544
K7-544
SeTau-425-Maleimide
SeTau-425-Maleimide
SH PB 7.4 425 4,200 545 39 26.2
K4-225
K4-225
Seta-385-NHS
Seta-385-NHS
NH2 PB 7.4 384 25,000 512 78
K6-5052
K6-5052
Seta-405 NHS
Seta-405 NHS
NH2 PB 7.4 401 30,000 452 70
K4-215
K4-215
Seta-400-NHS
Seta-400-NHS
NH2 PB 7.4 399 24,000 576 34
K9-3152
K9-3152
SeTau-488-NHS
SeTau-488-NHS
NH2 PBS 7.4 486 59,000 532 27
K9-4159
K9-4159 new
SeTau-660-NHS
SeTau-660-NHS
NH2 PB 7.4 663 240,000 694 50 3.36

Seta-405 is identical with Pacific Blue with excitation and emission at 401/452 nm.

SeTau-405 is a spectral analog of Pacific Orange with excitation and emission at 405/518 nm.

SeTau-488 with a similar absorption and emission as Alexa 488 (Φ = 0.92, ε = 73,000 and Φ. ε = 67,160) exhibits a lower brightness as a free dye but its brightness gets increased when it is attached to a biomolecule and it becomes an almost unquenchable fluorophore when labeled to an antibody (see specs sheet). Exc./Em. max of IgG-conjugates: 486/532 nm.

 

                                                   

 

SeTau-647 is an extremely bright label unsurpassed by any other red dye for excitation with the 633 nm laser. Its Q.Y. in aqueous solutions is 60% and its Stokes' shift is > 40 nm. It is currently one of the brightest fluorescent dyes for the red channel.

Seta-APC-705 and Seta-R-PE-775 are our latest editions to this product line.

Seta-APC-780 is a much brighter and a much more photostable replacement for APC-Cy7.

 Peripheral blood samples stained with CD81 — Seta-APC-780
 Peripheral blood samples stained with CD45 — Seta-APC-780
 Peripheral blood samples stained with CD3 — Seta-APC-780
Plasma cells stained with CD38 — Seta-APC-780

Top and bottom left: Peripheral blood samples stained with CD45-, CD81- and
CD3 — Seta-APC-780; Bottom right: plasma cells stained with CD38 — Seta-APC-780

Below is a table listing of our suggesteded new fluorophores/tandems for flow cytometry: 

 

Laser Source Labels Used Superior Seta Replacements

405 nm

Pacific Blue Seta-405-NHS
Pacific Orange SeTau-405-NHS

488 nm

Alexa 488 SeTau-488-NHS
R-PE - Cy5 Seta-R-PE-670
PerCP - Cy5.5 Seta-PerCP-680
 R-PE - Cy5.5 Seta-PerCP-680
R-PE - Cy7 Seta-R-PE-775

633 nm

APC, Alexa 647 SeTau-647-NHSSeta-650-NHS
 APC - Alexa 700 Seta-APC-705
Alexa 700 Seta-700-NHS
APC - Cy7 Seta-APC-780


For a specific listing and more detailed information about these tandems we refer you to tandem-conjugates.

Please contact us if you are interested in a sample for testing.

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