Flow Cytometry

Flow Cytometry is a powerful technique to measure multiple parameters from cell populations. Flow cytometers differentiate cells based on their size, internal complexity, and fluorescence signature. Qualitative and quantitative analyses of cell populations have relevance for clinical and research applications.

			Excitation Light Sources												Characteristics
Product Number (Specs Sheet)	Product Name (Product Info)	Target Group	380	405	436	488	532	594	635	650	680	700	750	780	Medium	λ abs [nm]	ε [M –1 cm–1]	λ em [nm]	QY [%]	FLT [ns]	Buy
K7-547 K7-547	SeTau-405-NHS SeTau-405-NHS	NH2	•	•											PB 7.4	405	13,800	518	80	9.3
K7-545 K7-545	SeTau-425-NHS SeTau-425-NHS	NH2	•	•	•										PB 7.4	425	4,200	545	39	26.2
K8-1384 K8-1384	Seta-700-NHS Seta-700-NHS	NH2	•	•						•	•	•			PB 7.4	687	177,000	703	6
K8-1682 K8-1682	Seta-660-di-NHS Seta-660-di-NHS	NH2							•	•					PB 7.4	661	220,000	672	11	0.9
K8-5035 K8-5035	Seta-650-NHS Seta-650-NHS	NH2							•	•					PB 7.4	651	200,000	671	28
K8-5036 K8-5036	Seta-650-Maleimide Seta-650-Maleimide	SH							•	•					PB 7.4	652	200,000	672	28
K8-7055 K8-7055	Seta-780-NHS Seta-780-NHS	NH2										•	•	•	PB 7.4	777	215,000	807	1.2
K9-4119 K9-4119	SeTau-665-NHS SeTau-665-NHS	NH2								•	•	•			PB 7.4	664	160,000	712	53	3.1
K9-4149 K9-4149	SeTau-647-NHS SeTau-647-NHS	NH2							•	•					PB 7.4	649	200,000	695	61	3.2
K8-1388 K8-1388	Seta-700-di-NHS Seta-700-di-NHS	NH2	•	•						•	•	•			PB 7.4	688	180,000	704	11
K8-1341 K8-1341	Seta-670-Maleimide Seta-670-Maleimide	SH	•	•					•	•					PB 7.4	667	180,000	688	7
K4-205 K4-205	Seta-375-NHS Seta-375-NHS	NH2	•												PB 7.4	372	24,500	476	86	3.1
K7-548 K7-548	SeTau-405-Maleimide SeTau-405-Maleimide	SH	•	•											PB 7.4	405	13,800	518	51	9.1
cl-APC cl-APC	Crosslinked APC Crosslinked APC							•	•	•					PB 7.4	650	700,000	662
R-PE R-PE	Lyophilized R-PE Lyophilized R-PE					•	•								PB 7.4	565	1,960,000	573
K10-6801 K10-6801	Seta-PerCP-680 Seta-PerCP-680				•	•	•								PB 7.4	481	380,000	676
K10-7801 K10-7801	Seta-APC-780 Seta-APC-780								•	•					PB 7.4	651	1,100,000	780
K10-6701 K10-6701	Seta-R-PE-670 Seta-R-PE-670					•	•								PB 7.4	488	1,100,000	671
K10-0775 K10-0775	Seta-R-PE-775 Seta-R-PE-775					•	•								PB 7.4	488	1,100,000	774
K10-7051 K10-7051	Seta-APC-705 Seta-APC-705								•	•					PB 7.4	655	1,100,000	703
K7-544 K7-544	SeTau-425-Maleimide SeTau-425-Maleimide	SH	•	•	•										PB 7.4	425	4,200	545	39	26.2
K4-225 K4-225	Seta-385-NHS Seta-385-NHS	NH2	•												PB 7.4	384	25,000	512	78
K6-5052 K6-5052	Seta-405 NHS Seta-405 NHS	NH2	•	•											PB 7.4	401	30,000	452	70
K4-215 K4-215	Seta-400-NHS Seta-400-NHS	NH2	•	•											PB 7.4	399	24,000	576	34
K9-3152 K9-3152	SeTau-488-NHS SeTau-488-NHS	NH2				•									PBS 7.4	486	59,000	532	27
K9-4159 K9-4159 new	SeTau-660-NHS SeTau-660-NHS	NH2							•	•					PB 7.4	663	240,000	694	50	3.36

Seta-405 is identical with Pacific Blue with excitation and emission at 401/452 nm.

SeTau-405 is a spectral analog of Pacific Orange with excitation and emission at 405/518 nm.

SeTau-488 with a similar absorption and emission as Alexa 488 (Φ = 0.92, ε = 73,000 and Φ. ε = 67,160) exhibits a lower brightness as a free dye but its brightness gets increased when it is attached to a biomolecule and it becomes an almost unquenchable fluorophore when labeled to an antibody (see specs sheet). Exc./Em. max of IgG-conjugates: 486/532 nm.

 

                                                   

 

SeTau-647 is an extremely bright label unsurpassed by any other red dye for excitation with the 633 nm laser. Its Q.Y. in aqueous solutions is 60% and its Stokes' shift is > 40 nm. It is currently one of the brightest fluorescent dyes for the red channel.

Seta-APC-705 and Seta-R-PE-775 are our latest editions to this product line.

Seta-APC-780 is a much brighter and a much more photostable replacement for APC-Cy7.

Below is a table listing of our suggesteded new fluorophores/tandems for flow cytometry: 

 

Laser Source	Labels Used	Superior Seta Replacements
405 nm	Pacific Blue	Seta-405-NHS
	Pacific Orange	SeTau-405-NHS
488 nm	Alexa 488	SeTau-488-NHS
	R-PE - Cy5	Seta-R-PE-670
	PerCP - Cy5.5	Seta-PerCP-680
	R-PE - Cy5.5	Seta-PerCP-680
	R-PE - Cy7	Seta-R-PE-775
633 nm
	APC, Alexa 647	SeTau-647-NHS, Seta-650-NHS
	APC - Alexa 700	Seta-APC-705
	Alexa 700	Seta-700-NHS
	APC - Cy7	Seta-APC-780

For a specific listing and more detailed information about these tandems we refer you to tandem-conjugates.

Please contact us if you are interested in a sample for testing.